Up-regulation of IL-6-receptors by IL-3 on a plasma cell leukaemia cell line which proliferates dependently on both IL-3 and IL-6

M Kobayashi; J Tanaka; M Imamura; S Maeda; H Iwasaki; M Tanaka; Y Tsudu; K Sakurada; T Miyazaki

doi:10.1111/j.1365-2141.1993.tb04687.x

Up-regulation of IL-6-receptors by IL-3 on a plasma cell leukaemia cell line which proliferates dependently on both IL-3 and IL-6

Br J Haematol. 1993 Apr;83(4):535-8. doi: 10.1111/j.1365-2141.1993.tb04687.x.

Authors

M Kobayashi¹, J Tanaka, M Imamura, S Maeda, H Iwasaki, M Tanaka, Y Tsudu, K Sakurada, T Miyazaki

Affiliation

¹ Laboratory of Pathology, Cancer Institute, Hokkaido University School of Medicine, Sapporo, Japan.

PMID: 8518172
DOI: 10.1111/j.1365-2141.1993.tb04687.x

Abstract

In this report we show that IL-3 up-regulates the expression of IL-6-receptors on a plasma cell leukaemia cell line which proliferates in response to both IL-3 and IL-6. Quantitative binding studies and Scatchard analysis revealed that HSM-2.3 has a single class of high-affinity IL-6 binding sites (200/cell, dissociation constant at the equilibrium 1.43 x 10(-11) M). After stimulation with IL-3, HSM-2.3 has 1200 binding sites. IL-6 receptor mRNA expression was detected in HSM-2.3 only after stimulation with IL-3.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Humans
Interleukin-3 / immunology*
Interleukin-6 / genetics
Interleukin-6 / immunology*
Leukemia, Plasma Cell / immunology*
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Messenger / analysis
Receptors, Immunologic / analysis*
Receptors, Immunologic / genetics
Receptors, Interleukin-6
Tumor Cells, Cultured / immunology
Up-Regulation

Substances

Interleukin-3
Interleukin-6
RNA, Messenger
Receptors, Immunologic
Receptors, Interleukin-6