Abstract
Activation of phosphatidylcholine-specific phospholipase D (PLD) has been implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. We report here the identification of the first human PLD cDNA, which defines a new and highly conserved gene family. Characterization of recombinant human PLD1 reveals that it is membrane-associated, selective for phosphatidylcholine, stimulated by phosphatidylinositol 4,5-bisphosphate, activated by the monomeric G-protein ADP-ribosylation factor-1, and inhibited by oleate. PLD1 likely encodes the gene product responsible for the most widely studied endogenous PLD activity.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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ADP-Ribosylation Factors
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Amino Acid Sequence
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Animals
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Bacteria / enzymology
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Baculoviridae
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Caenorhabditis elegans / enzymology
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Carrier Proteins / metabolism
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Cell Line
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Chlorocebus aethiops
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Cloning, Molecular
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Conserved Sequence
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Enzyme Activation
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GTP-Binding Proteins / metabolism*
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Humans
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Kinetics
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Mice
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Molecular Sequence Data
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Multigene Family*
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Phosphatidylcholines / metabolism
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Phosphatidylinositol 4,5-Diphosphate
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Phosphatidylinositol Phosphates / pharmacology
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Phospholipase D / genetics*
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Phospholipase D / metabolism
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Plants / enzymology
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Recombinant Proteins / metabolism
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Spodoptera
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Substrate Specificity
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Transfection
Substances
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Carrier Proteins
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Phosphatidylcholines
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Phosphatidylinositol 4,5-Diphosphate
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Phosphatidylinositol Phosphates
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Recombinant Proteins
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Phospholipase D
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GTP-Binding Proteins
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ADP-Ribosylation Factors
Associated data
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GENBANK/D27058
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GENBANK/D33536
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GENBANK/G00778
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GENBANK/L33686
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GENBANK/T76232
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GENBANK/T88610
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GENBANK/U38545
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GENBANK/X28256
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GENBANK/Z18424
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GENBANK/Z33674