Abstract
Recombinant human T-cell leukemia virus type II (HTLV-II) envelope external glycoprotein, gp46-II, was expressed using a vaccinia virus vector. A recombinant gp46-II fused to an epitope of the influenza virus hemagglutinin, YPYDVPDYA, was purified by immunoaffinity chromatography. The purified glycoprotein was used to immunize Balb/c mice, and antibodies against gp46-II were detected by Western blot analysis and syncytium inhibition assays. We transformed spleen cells from the immunized mice by retroviral infection with ABL-MYC (psi 2) and intraperitoneally transplanted the infected cells into syngeneic Balb/c and severe combined immunodeficient (SCID) mice. The plasmacytomas established ascitic tumors that produced antibodies directed against HTLV-II gp46-II. Ascites developed more rapidly in SCID mice than in normal syngeneic mice. This procedure provides a general means to generate antibodies rapidly.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Antibodies, Viral / biosynthesis*
-
Antibodies, Viral / immunology
-
Cell Line
-
Cell Transformation, Viral
-
Deltaretrovirus Antigens / genetics
-
Deltaretrovirus Antigens / immunology*
-
Gene Products, env / genetics
-
Gene Products, env / immunology*
-
Hemagglutinin Glycoproteins, Influenza Virus
-
Hemagglutinins, Viral / genetics
-
Hemagglutinins, Viral / metabolism
-
Humans
-
Mice
-
Mice, Inbred BALB C
-
Mice, SCID
-
Molecular Sequence Data
-
Plasmacytoma / immunology*
-
Recombinant Fusion Proteins / immunology
-
Retroviridae Proteins, Oncogenic / genetics
-
Retroviridae Proteins, Oncogenic / immunology*
-
Spleen / cytology
-
env Gene Products, Human Immunodeficiency Virus
Substances
-
Antibodies, Viral
-
Deltaretrovirus Antigens
-
Gene Products, env
-
Hemagglutinin Glycoproteins, Influenza Virus
-
Hemagglutinins, Viral
-
Recombinant Fusion Proteins
-
Retroviridae Proteins, Oncogenic
-
env Gene Products, Human Immunodeficiency Virus
-
gp46 protein, Human immunodeficiency virus 2