Rapid generation of antibodies against the HTLV-II external envelope protein by growth of mouse plasmacytomas in SCID mice

Virology. 1995 Dec 20;214(2):680-4. doi: 10.1006/viro.1995.0085.

Abstract

Recombinant human T-cell leukemia virus type II (HTLV-II) envelope external glycoprotein, gp46-II, was expressed using a vaccinia virus vector. A recombinant gp46-II fused to an epitope of the influenza virus hemagglutinin, YPYDVPDYA, was purified by immunoaffinity chromatography. The purified glycoprotein was used to immunize Balb/c mice, and antibodies against gp46-II were detected by Western blot analysis and syncytium inhibition assays. We transformed spleen cells from the immunized mice by retroviral infection with ABL-MYC (psi 2) and intraperitoneally transplanted the infected cells into syngeneic Balb/c and severe combined immunodeficient (SCID) mice. The plasmacytomas established ascitic tumors that produced antibodies directed against HTLV-II gp46-II. Ascites developed more rapidly in SCID mice than in normal syngeneic mice. This procedure provides a general means to generate antibodies rapidly.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Viral / biosynthesis*
  • Antibodies, Viral / immunology
  • Cell Line
  • Cell Transformation, Viral
  • Deltaretrovirus Antigens / genetics
  • Deltaretrovirus Antigens / immunology*
  • Gene Products, env / genetics
  • Gene Products, env / immunology*
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Hemagglutinins, Viral / genetics
  • Hemagglutinins, Viral / metabolism
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Mice, SCID
  • Molecular Sequence Data
  • Plasmacytoma / immunology*
  • Recombinant Fusion Proteins / immunology
  • Retroviridae Proteins, Oncogenic / genetics
  • Retroviridae Proteins, Oncogenic / immunology*
  • Spleen / cytology
  • env Gene Products, Human Immunodeficiency Virus

Substances

  • Antibodies, Viral
  • Deltaretrovirus Antigens
  • Gene Products, env
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Hemagglutinins, Viral
  • Recombinant Fusion Proteins
  • Retroviridae Proteins, Oncogenic
  • env Gene Products, Human Immunodeficiency Virus
  • gp46 protein, Human immunodeficiency virus 2