Use of a new oligonucleotide probe for detection of colonization factor antigen III gene in enterotoxigenic Escherichia coli

Eur J Clin Microbiol Infect Dis. 1995 Aug;14(8):713-6. doi: 10.1007/BF01690882.

Abstract

An alkaline phosphatase-labeled 30-mer oligonucleotide probe was designed to detect the gene for pilus colonization factor antigen III (CFA/III) of the human type of enterotoxigenic Escherichia coli (ETEC). The CFA/III probe was used to identify CFA/III-producing ETEC among 303 Escherichia coli obtained from subjects with traveler's diarrhea. Six isolates positive for the CFA/III gene were found. This result was confirmed immunologically by using a specific monoclonal antibody developed against CFA/III. These six isolates, isolated from travelers returning from India, Pakistan and China, were all positive for the gene of heat-labile enterotoxin and possessed an identical serotype (025:H-).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Bacterial / analysis
  • Antigens, Bacterial / genetics*
  • Bacterial Proteins / analysis
  • Bacterial Proteins / genetics*
  • Bacterial Typing Techniques
  • Base Sequence
  • China
  • Diarrhea / microbiology*
  • Escherichia coli / genetics
  • Escherichia coli / immunology*
  • Fimbriae Proteins*
  • Humans
  • India
  • Molecular Sequence Data
  • Oligonucleotide Probes*
  • Pakistan
  • Sensitivity and Specificity
  • Travel*

Substances

  • Antigens, Bacterial
  • Bacterial Proteins
  • Oligonucleotide Probes
  • colonization factor antigens
  • Fimbriae Proteins