Cloning, chromosomal localization, and tissue expression of autotaxin from human teratocarcinoma cells

Biochem Biophys Res Commun. 1996 Jan 26;218(3):714-9. doi: 10.1006/bbrc.1996.0127.

Abstract

Autotaxin, a potent human tumor cell motility-stimulating exophosphodiesterase, was isolated and cloned from the human teratocarcinoma cell line NTera2D1. The deduced amino acid sequence for the teratocarcinoma autotaxin has 94% identity to the melanoma-derived protein, 90% identity to rat brain phosphodiesterase I/nucleotide pyrophosphatase (PD-I alpha), and 44% identity to the plasma cell membrane marker PC-I. Utilizing polymerase chain reaction screening of the CEPH YAC library, we localized the autotaxin gene to human chromosome 8q23-24. Northern blot analysis of relative mRNA from multiple human tissues revealed that autotaxin mRNA steady state expression is most abundant in brain, placenta, ovary, and small intestine.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Movement
  • Chromosome Mapping
  • Chromosomes, Artificial, Yeast
  • Chromosomes, Human, Pair 8
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Gene Expression
  • Genes
  • Glucose-6-Phosphate Isomerase / genetics*
  • Glycoproteins / genetics*
  • Humans
  • Melanoma / chemistry
  • Molecular Sequence Data
  • Multienzyme Complexes*
  • Phosphodiesterase I
  • Phosphoric Diester Hydrolases
  • Pyrophosphatases
  • RNA, Messenger / genetics
  • Rats
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Teratocarcinoma / chemistry

Substances

  • DNA, Complementary
  • Glycoproteins
  • Multienzyme Complexes
  • RNA, Messenger
  • Phosphoric Diester Hydrolases
  • Phosphodiesterase I
  • alkylglycerophosphoethanolamine phosphodiesterase
  • Pyrophosphatases
  • Glucose-6-Phosphate Isomerase

Associated data

  • GENBANK/L46720