Abstract
We have analyzed 3.4 kb of DNA from the 5'-flanking region of the rat beta 2-adrenergic receptor gene and assessed its promoter activity in A549 cells, a human lung adenocarcinoma cell line. A single transcription start site was identified approx. 223 bp upstream of the ATG start codon. A549 cells were transfected with luciferase reporter plasmids containing segments of the rat beta 2-adrenergic receptor 5'-flanking region. Our results suggest that both positive and negative cis-acting regulatory sequences are present in the 5'-flanking region of the rat beta 2-adrenergic receptor gene.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenocarcinoma
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Amino Acid Sequence
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Animals
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Base Sequence
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Binding Sites
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Cell Line
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Codon
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DNA / chemistry
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DNA / isolation & purification
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DNA Primers
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DNA-Binding Proteins / metabolism
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Humans
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Luciferases / biosynthesis
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Lung Neoplasms
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Molecular Sequence Data
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Molecular Weight
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Promoter Regions, Genetic*
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Rats
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Receptors, Adrenergic, beta-2 / genetics*
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Recombinant Proteins / biosynthesis
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Restriction Mapping
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TATA Box
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Transcription, Genetic
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Transfection
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Tumor Cells, Cultured
Substances
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Codon
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DNA Primers
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DNA-Binding Proteins
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Receptors, Adrenergic, beta-2
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Recombinant Proteins
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DNA
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Luciferases