Abstract
The presence of BCL-2 gene rearrangement has been detected also in cellular populations lacking the t(14;18) chromosomal translocation, such as B-lineage acute lymphoblastic leukemia (ALL) cells. It has been reported that overexpression of BCL-2 is related to resistance to glucocorticoid-induced apoptosis. In this study, we aimed to evaluate whether in vitro culture with prednisolone (PDN) could modify the expression of BCL-2 protein. ALL cells from 21 patients were incubated for 72 hr with or without a minimally lethal (IC12) dose of PDN. In vitro culture with PDN did not affect the percentage of positive cells, even though the mean fluorescence index was significantly increased (P = 0.0001), thus indicating a higher level of protein production. These data could suggest a possible mechanism of drug resistance after treatment with PDN.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Aged
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Antineoplastic Combined Chemotherapy Protocols / therapeutic use
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Apoptosis / drug effects
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Asparaginase / administration & dosage
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Bone Marrow / pathology
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Burkitt Lymphoma / drug therapy
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Burkitt Lymphoma / genetics
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Burkitt Lymphoma / metabolism
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Burkitt Lymphoma / mortality
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Burkitt Lymphoma / pathology*
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Cyclophosphamide / administration & dosage
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Doxorubicin / administration & dosage
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Drug Resistance, Neoplasm / genetics
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Female
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Gene Expression Regulation, Leukemic / drug effects*
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Humans
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Male
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Middle Aged
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Neoplasm Proteins / biosynthesis*
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Neoplasm Proteins / genetics
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Prednisolone / administration & dosage
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Prednisolone / pharmacology*
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Proto-Oncogene Proteins / biosynthesis*
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins c-bcl-2
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Tumor Cells, Cultured / drug effects
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Vincristine / administration & dosage
Substances
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Neoplasm Proteins
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-bcl-2
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Vincristine
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Doxorubicin
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Cyclophosphamide
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Prednisolone
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Asparaginase