Abstract
We have utilized in situ hybridization and immunocytochemistry techniques to examine the expression of both alpha 2-macroglobulin (alpha 2-MR) and the 39 kDa receptor-associated protein (RAP) in 8 benign (BPH) and 34 malignant human prostate tissues, including 4 metastases. The levels of alpha 2-MR mRNA expression (but not RAP) increased significantly in high Gleason score carcinomas ( > 8) and in metastatic lesions, suggesting that alpha 2-MR expression is associated with advanced cancer. Semi-quantitative analysis with computer-assisted system analysis (CASA) confirmed this interpretation. This is the first report of alpha 2-MR expression being associated with advanced prostate cancer.
MeSH terms
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Base Sequence
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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DNA Probes
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Gene Expression Regulation, Neoplastic
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Glycoproteins / genetics
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Glycoproteins / metabolism*
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Humans
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Immunohistochemistry
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In Situ Hybridization
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LDL-Receptor Related Protein-Associated Protein
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Male
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Molecular Sequence Data
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Oligonucleotides, Antisense / genetics
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Oligoribonucleotides / genetics
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Prostatic Hyperplasia / metabolism*
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Prostatic Neoplasms / chemistry*
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Prostatic Neoplasms / pathology
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RNA, Messenger / analysis
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RNA, Neoplasm / analysis
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Receptors, Immunologic / analysis*
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Up-Regulation
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alpha-Macroglobulins / metabolism*
Substances
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Carrier Proteins
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DNA Probes
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Glycoproteins
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LDL-Receptor Related Protein-Associated Protein
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Oligonucleotides, Antisense
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Oligoribonucleotides
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RNA, Messenger
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RNA, Neoplasm
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Receptors, Immunologic
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alpha-Macroglobulins