Abstract
Hel-N1 and HuD are neuron-specific RNA-binding proteins that are antigenic targets of anti-Hu antibodies. Although expression of Hu antigens is most commonly seen in small-cell lung carcinoma, their exact identity (e.g., Hel-NI, Hel-N2, HuD, and HuC cannot be distinguished by immunological methods. Analysis of messenger RNA expression is needed for this distinction. Here we demonstrate that Hel-NI and Hel-N2 are expressed in small-cell lung carcinoma using reverse transcription-polymerase chain reaction.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Carcinoma, Small Cell / genetics*
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DNA Primers / genetics
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ELAV Proteins
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ELAV-Like Protein 2
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ELAV-Like Protein 4
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Gene Expression Regulation, Neoplastic / physiology
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Humans
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Isomerism
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Leukemia, Erythroblastic, Acute
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Lung Neoplasms / genetics*
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Molecular Sequence Data
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Nerve Tissue Proteins / genetics*
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Polymerase Chain Reaction
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RNA-Binding Proteins / genetics*
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Tumor Cells, Cultured / physiology
Substances
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DNA Primers
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ELAV Proteins
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ELAV-Like Protein 2
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ELAV-Like Protein 4
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ELAVL2 protein, human
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ELAVL4 protein, human
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Nerve Tissue Proteins
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RNA-Binding Proteins