Abstract
Through the specific binding of a negative calcium-responsive element to its binding protein in response to extracellular Ca (Ca2+e), negative calcium-responsive element-bearing genes, such as the human parathyroid hormone gene, are negatively regulated by Ca2+e. The Ku antigen mediated negative gene regulation by Ca2+e by interacting with a redox factor protein, REF1. Although sequence-nonspecific DNA binding activity of the Ku antigen has been well characterized, the mechanism of its sequence-specific DNA binding remained obscure. Here, we report that the specific binding of the Ku antigen to another protein, REF1, leads to DNA-protein complex formation with a novel sequence specificity and thereby regulates gene expression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antigens, Nuclear*
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Base Sequence
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Binding Sites
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Calcium / metabolism*
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Carbon-Oxygen Lyases*
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DNA Helicases*
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DNA-(Apurinic or Apyrimidinic Site) Lyase*
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DNA-Binding Proteins / metabolism*
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Gene Expression Regulation
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HeLa Cells
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Humans
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Ku Autoantigen
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Macromolecular Substances
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Molecular Sequence Data
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Nuclear Proteins / metabolism*
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Oligodeoxyribonucleotides / chemistry
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Protein Binding
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Repressor Proteins / metabolism
Substances
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Antigens, Nuclear
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DNA-Binding Proteins
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Macromolecular Substances
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Nuclear Proteins
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Oligodeoxyribonucleotides
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Repressor Proteins
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DNA Helicases
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XRCC5 protein, human
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Xrcc6 protein, human
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Carbon-Oxygen Lyases
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Ku Autoantigen
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APEX1 protein, human
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DNA-(Apurinic or Apyrimidinic Site) Lyase
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Calcium