Abstract
MLL is fused to ENL or ELL in acute leukemias that contain t(ll;19)(q23;p13). Although ENL and ELL localize to chromosome 19, bands p13.3 and p13.1, respectively, these breakpoints are not always readily distinguished by standard cytogenetics. We therefore used reverse transcriptase-polymerase chain reaction (RT-PCR) assays to analyze 26 cases of childhood acute leukemia containing t(11;19) to determine the frequencies of ENL and ELL involvement. All 17 cases of acute lymphoblastic leukemia (ALL) had MLL/ENL fusion transcripts. By contrast, of the 9 cases of acute myeloid leukemia (AML) analyzed, 6 had MLL/ENL fusions, 2 had MLL/ELL fusions, and 1 case had no RT-PCR-detectable MLL fusion mRNA. These data suggest that the majority of 11;19 translocations involve ENL, whereas involvement of ELL is relatively uncommon in childhood acute leukemia and may be restricted to AML.
MeSH terms
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Acute Disease
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Adolescent
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Base Sequence
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Burkitt Lymphoma / genetics
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Burkitt Lymphoma / pathology
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Child
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Child, Preschool
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Chromosomes, Human, Pair 11 / genetics
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Chromosomes, Human, Pair 11 / ultrastructure*
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Chromosomes, Human, Pair 19 / genetics
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Chromosomes, Human, Pair 19 / ultrastructure*
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DNA, Neoplasm / genetics
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DNA-Binding Proteins / genetics*
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Female
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Histone-Lysine N-Methyltransferase
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Humans
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Infant
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Infant, Newborn
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Leukemia / genetics*
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Leukemia / pathology
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Leukemia, Myeloid / genetics
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Leukemia, Myeloid / pathology
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Leukemia-Lymphoma, Adult T-Cell / genetics
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Leukemia-Lymphoma, Adult T-Cell / pathology
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Male
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Molecular Sequence Data
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Myeloid-Lymphoid Leukemia Protein
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Neoplasm Proteins*
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Neoplastic Stem Cells / pathology
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Nuclear Proteins / genetics*
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Oncogene Proteins, Fusion / genetics*
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Peptide Elongation Factors*
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Precursor Cell Lymphoblastic Leukemia-Lymphoma / genetics
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Precursor Cell Lymphoblastic Leukemia-Lymphoma / pathology
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Proto-Oncogenes*
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Transcription Factors / genetics*
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Transcriptional Elongation Factors
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Translocation, Genetic*
Substances
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DNA, Neoplasm
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DNA-Binding Proteins
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ELL protein, human
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KMT2A protein, human
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MLLT1 protein, human
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Neoplasm Proteins
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Nuclear Proteins
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Oncogene Proteins, Fusion
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Peptide Elongation Factors
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Transcription Factors
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Transcriptional Elongation Factors
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Myeloid-Lymphoid Leukemia Protein
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Histone-Lysine N-Methyltransferase