Forced over-expression of the myeloid zinc finger gene MZF-1 inhibits apoptosis and promotes oncogenesis in interleukin-3-dependent FDCP.1 cells

R Hromas; S Boswell; R N Shen; G Burgess; A Davidson; K Cornetta; J Sutton; K Robertson

Forced over-expression of the myeloid zinc finger gene MZF-1 inhibits apoptosis and promotes oncogenesis in interleukin-3-dependent FDCP.1 cells

Leukemia. 1996 Jun;10(6):1049-50.

Authors

R Hromas¹, S Boswell, R N Shen, G Burgess, A Davidson, K Cornetta, J Sutton, K Robertson

Affiliation

¹ Division of Hematology/Oncology, Indiana University Medical Center, Indianapolis 46202, USA.

PMID: 8667641

Abstract

The myeloid zinc finger protein MZF-1 is important in hematopoiesis. Previous studies have found that reducing expression of MZF-1 inhibited G-CSF-driven human marrow colony formation assays. In this study we found that retrovirally overexpressing MZF-1 in IL-3-dependent FDCP.1 cells inhibited their apoptosis when IL-3 was withdrawn. The MZF-1-transduced FDCP.1 cells also formed tumors when injected into congenic mice, whereas control FDCP.1 cells did not.

MeSH terms

Animals
Apoptosis / genetics*
Bone Marrow / metabolism*
Cell Transformation, Neoplastic / genetics*
Cells, Cultured
DNA-Binding Proteins / genetics*
Gene Expression*
Humans
Interleukin-3 / pharmacology*
Kruppel-Like Transcription Factors
Mice
Neoplasm Transplantation
Transcription Factors / genetics*
Zinc Fingers / genetics*

Substances

DNA-Binding Proteins
Interleukin-3
Kruppel-Like Transcription Factors
MZF1 protein, human
Mzf1 protein, mouse
Transcription Factors