Abstract
The exopolysaccharide produced by Lactobacillus acidophilus LMG9433 in a semi-defined medium was found to be a charged heteropolymer, with a composition of D-glucose, D-galactose, D-glucuronic acid, and 2-acetamido-2-deoxy-D-glucose in molar ratios of 2:1:1:1. By means of methylation analysis, uronic acid degradation, de-N-acetylation/deamination, partial acid hydrolysis, and 1D/2D NMR studies the polysaccharide was demonstrated to consist of repeating units with the following structure: [Table: see text]
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carbohydrate Conformation
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Carbohydrate Sequence
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Chromatography, High Pressure Liquid
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Hydrolysis
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Lactobacillus acidophilus / chemistry*
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Magnetic Resonance Spectroscopy
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Methylation
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Molecular Sequence Data
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Monosaccharides / analysis
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Oligosaccharides / analysis
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Oligosaccharides / chemistry
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Polysaccharides, Bacterial / chemistry*
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Polysaccharides, Bacterial / isolation & purification
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Uronic Acids
Substances
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Monosaccharides
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Oligosaccharides
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Polysaccharides, Bacterial
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Uronic Acids