Structural characterization of the exopolysaccharide produced by Lactobacillus acidophilus LMG9433

Carbohydr Res. 1996 Jul 19:288:203-18. doi: 10.1016/s0008-6215(96)90799-5.

Abstract

The exopolysaccharide produced by Lactobacillus acidophilus LMG9433 in a semi-defined medium was found to be a charged heteropolymer, with a composition of D-glucose, D-galactose, D-glucuronic acid, and 2-acetamido-2-deoxy-D-glucose in molar ratios of 2:1:1:1. By means of methylation analysis, uronic acid degradation, de-N-acetylation/deamination, partial acid hydrolysis, and 1D/2D NMR studies the polysaccharide was demonstrated to consist of repeating units with the following structure: [Table: see text]

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Chromatography, High Pressure Liquid
  • Hydrolysis
  • Lactobacillus acidophilus / chemistry*
  • Magnetic Resonance Spectroscopy
  • Methylation
  • Molecular Sequence Data
  • Monosaccharides / analysis
  • Oligosaccharides / analysis
  • Oligosaccharides / chemistry
  • Polysaccharides, Bacterial / chemistry*
  • Polysaccharides, Bacterial / isolation & purification
  • Uronic Acids

Substances

  • Monosaccharides
  • Oligosaccharides
  • Polysaccharides, Bacterial
  • Uronic Acids