Interaction of the c-cbl proto-oncogene product with the Tyk-2 protein tyrosine kinase

Biochem Biophys Res Commun. 1996 Aug 23;225(3):833-8. doi: 10.1006/bbrc.1996.1259.

Abstract

The c-cbl proto-oncogene product (p120cbl) forms a stable complex with the Tyk-2 protein tyrosine kinase in various human cell lines of diverse hematopoietic origin. In U-266 myeloma and 293T embryonic kidney cells, p120cbl is rapidly phosphorylated on tyrosine in an IFN alpha-dependent manner. p120cbl also acts as a specific substrate for the Tyk-2-associated SHP-1 phosphatase in vitro, suggesting that this phosphatase plays a regulatory role on the phosphorylation of the protein. These data provide evidence that p120cbl interacts with the functional Type I IFN receptor complex, and suggest its involvement in IFN alpha signaling.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cell Line
  • Humans
  • Interferon-alpha / metabolism
  • Intracellular Signaling Peptides and Proteins
  • Phosphorylation
  • Protein Tyrosine Phosphatase, Non-Receptor Type 11
  • Protein Tyrosine Phosphatase, Non-Receptor Type 6
  • Protein Tyrosine Phosphatases / metabolism
  • Protein-Tyrosine Kinases / metabolism*
  • Proteins / metabolism*
  • Proto-Oncogene Mas
  • Proto-Oncogene Proteins / metabolism*
  • Proto-Oncogene Proteins c-cbl
  • Receptor, Interferon alpha-beta
  • Receptors, Interferon / metabolism
  • Signal Transduction
  • Substrate Specificity
  • TYK2 Kinase
  • Ubiquitin-Protein Ligases*

Substances

  • Interferon-alpha
  • Intracellular Signaling Peptides and Proteins
  • MAS1 protein, human
  • Proteins
  • Proto-Oncogene Mas
  • Proto-Oncogene Proteins
  • Receptors, Interferon
  • Receptor, Interferon alpha-beta
  • Proto-Oncogene Proteins c-cbl
  • Ubiquitin-Protein Ligases
  • Protein-Tyrosine Kinases
  • TYK2 Kinase
  • TYK2 protein, human
  • PTPN11 protein, human
  • PTPN6 protein, human
  • Protein Tyrosine Phosphatase, Non-Receptor Type 11
  • Protein Tyrosine Phosphatase, Non-Receptor Type 6
  • Protein Tyrosine Phosphatases
  • CBL protein, human