Abstract
We have cloned the rhesus monkey obese cDNA and have analyzed its expression in monkeys with a wide range of body weights (lean to very obese) and with or without non-insulin-dependent diabetes mellitus to examine the relationship of ob gene expression to obesity and non-insulin-dependent diabetes mellitus. The sequence of monkey ob protein, excluding the signal peptide, showed 91% identity with the human protein. We observed a significant correlation between the level of ob mRNA and body weight. We also found a significant relationship between ob mRNA and fasting plasma insulin concentration; however, insulin stimulation during a 100-140-min euglycemic/hyperinsulinemic clamp did not result in any changes in ob mRNA levels. Circulating levels of the ob gene product leptin were also significantly correlated with body weight. These results show that ob gene expression is related to body weight and is not acutely regulated by insulin.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Body Weight
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DNA, Complementary
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Diabetes Mellitus / genetics
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Diabetes Mellitus / physiopathology
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Diabetes Mellitus / veterinary*
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Diabetes Mellitus, Type 2 / genetics
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Diabetes Mellitus, Type 2 / physiopathology
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Diabetes Mellitus, Type 2 / veterinary*
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Gene Expression Regulation*
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Humans
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Hyperinsulinism / genetics
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Hyperinsulinism / physiopathology
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Hyperinsulinism / veterinary
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Leptin
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Macaca mulatta
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Mice
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Molecular Sequence Data
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Obesity / genetics
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Obesity / physiopathology
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Obesity / veterinary*
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Organ Specificity
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Primate Diseases*
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Protein Biosynthesis*
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Protein Sorting Signals / biosynthesis
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Proteins / metabolism
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RNA, Messenger / biosynthesis
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Rats
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Regression Analysis
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Sequence Homology, Amino Acid
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Transcription, Genetic*
Substances
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DNA, Complementary
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Leptin
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Protein Sorting Signals
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Proteins
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RNA, Messenger