Abstract
We have investigated the relationship between XPA gene mutations and PCNA complex formation in the nucleotide excision repair (NER) process utilizing cells derived from various xeroderma pigmentosum group A (XP-A) patients. The PCNA complex formation was detected by PCNA immunostaining following methanol fixation. Results indicated that UV-induced PCNA staining at early stages was well correlated to the function of XPA protein and provided evidence that XPA protein-related recognition step was tightly linked to PCNA-associated events in the NER process in vivo.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line
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Codon
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DNA / radiation effects
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DNA Damage
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DNA Repair
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DNA-Binding Proteins / biosynthesis
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DNA-Binding Proteins / genetics*
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Exons
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Fibroblasts
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Genetic Carrier Screening
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Homozygote
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Humans
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Immunohistochemistry
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Mutation*
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Point Mutation
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Proliferating Cell Nuclear Antigen / analysis
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Proliferating Cell Nuclear Antigen / biosynthesis*
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Sequence Deletion
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Ultraviolet Rays*
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Xeroderma Pigmentosum / genetics*
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Xeroderma Pigmentosum Group A Protein
Substances
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Codon
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DNA-Binding Proteins
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Proliferating Cell Nuclear Antigen
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XPA protein, human
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Xeroderma Pigmentosum Group A Protein
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DNA