Abstract
Pneumocystis carinii pneumonia (PCP) is usually diagnosed by examination of BAL, a sample often unpleasant to be collected from immunocompromised host affected by acute respiratory disease. We studied by the Internal Transcribed Spacers (ITSs) nested PCR the presence of P.carinii DNA in serum and Peripheral Blood Mononuclear Cells (PBMC) during acute episodes of PCP to test blood as a possible noninvasive diagnostic tool.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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AIDS-Related Opportunistic Infections / blood
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AIDS-Related Opportunistic Infections / microbiology*
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DNA, Fungal / analysis*
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Humans
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Leukocytes, Mononuclear / microbiology*
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Pneumocystis / genetics
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Pneumocystis / isolation & purification*
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Pneumonia, Pneumocystis / blood
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Pneumonia, Pneumocystis / complications
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Pneumonia, Pneumocystis / microbiology*
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Polymerase Chain Reaction / methods*