Abstract
Tumor cells frequently express the insulin-like growth factor I (IGF-I). Recently we demonstrated that rat glioma cells when transfected with a vector encoding antisense IGF-I cDNA lost tumorigenicity and induced a tumor specific immune response involving CD8+ lymphocytes. Here we show that the cultured transfected cell lines, rat C-6 glioma, human primary glioma and mouse teratocarcinoma, expressed an increased level of MHC-I and of co-signaling B-7 molecules. This increased expression of MHC-I and B-7, demonstrated by 51Cr release complement dependent cytoxicity assay and by immunostaining flow cytometry analysis, could contribute to the final immune recognition of glioma immunogenicity.
MeSH terms
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Animals
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Antigens, CD / analysis*
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B7-1 Antigen / analysis*
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B7-2 Antigen
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Flow Cytometry
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Gene Expression / physiology
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Glioma
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Histocompatibility Antigens Class I / analysis*
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Humans
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Insulin-Like Growth Factor I / genetics*
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Insulin-Like Growth Factor II / genetics
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Membrane Glycoproteins / analysis*
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Mice
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Neuroblastoma
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RNA, Antisense
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RNA, Messenger / metabolism
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Rats
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Teratocarcinoma
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Transfection
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Tumor Cells, Cultured / chemistry
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Tumor Cells, Cultured / physiology
Substances
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Antigens, CD
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B7-1 Antigen
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B7-2 Antigen
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CD86 protein, human
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Cd86 protein, mouse
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Cd86 protein, rat
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Histocompatibility Antigens Class I
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Membrane Glycoproteins
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RNA, Antisense
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RNA, Messenger
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Insulin-Like Growth Factor I
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Insulin-Like Growth Factor II