Abstract
Monocytes as well as lymphocytes infiltrate in the stroma of thyroid tissue in autoimmune and destructive thyroiditis. Monocyte chemoattractant protein-1 (MCP-1) is a cytokine that attracts T-lymphocytes as well as monocytes. Using human thyrocytes in primary cultures, we show that expression of MCP-1 mRNA and protein is remarkably stimulated by both interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha), and also that interferon-gamma (IFN-gamma) by itself is a weak stimulant but has a synergistic activity with either IL-1 or TNF-alpha. The finding indicates that MCP-1 can be produced by thyrocytes themselves, suggesting a possible role of thyrocytes on accumulation of monocytes and T-lymphocytes to the tissue from the blood in autoimmune and destructive thyroiditis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cells, Cultured
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Chemokine CCL2 / biosynthesis*
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Chemokine CCL2 / genetics
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Cycloheximide / pharmacology
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DNA, Complementary
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Dactinomycin / pharmacology
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Dexamethasone / pharmacology
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Electrophoresis, Agar Gel
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Graves Disease / metabolism*
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Humans
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Interferon-gamma / pharmacology
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Interleukin-1 / pharmacology
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Polymerase Chain Reaction
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Thyroglobulin / metabolism
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Thyroid Gland / cytology
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Thyroid Gland / metabolism*
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Thyroid Gland / pathology
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Thyrotropin / pharmacology
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Tumor Necrosis Factor-alpha / pharmacology
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Up-Regulation / physiology
Substances
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Chemokine CCL2
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DNA, Complementary
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Interleukin-1
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RNA, Messenger
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Tumor Necrosis Factor-alpha
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Dactinomycin
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Dexamethasone
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Interferon-gamma
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Thyrotropin
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Thyroglobulin
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Cycloheximide