Deletion of exon b3 of the BCR gene in CML: easy breakpoint mapping by a two-round PCR

J C Cigudosa; M T Acosta Almeida; J L Carrasco Juan; A Otero Gómez; L Hernández Nieto; J García Talavera; J L García Miranda

doi:10.1016/s0165-4608(96)00111-2

Deletion of exon b3 of the BCR gene in CML: easy breakpoint mapping by a two-round PCR

Cancer Genet Cytogenet. 1996 Oct 1;91(1):71-3. doi: 10.1016/s0165-4608(96)00111-2.

Authors

J C Cigudosa¹, M T Acosta Almeida, J L Carrasco Juan, A Otero Gómez, L Hernández Nieto, J García Talavera, J L García Miranda

Affiliation

¹ Service of Genetics, Faculty of Medicine, University of La Laguna, Tenerife, Spain.

PMID: 8908171
DOI: 10.1016/s0165-4608(96)00111-2

Abstract

We have performed the molecular analysis for the detection of the BCR-ABL and ABL-BCR fusion genes in 50 patients with myeloproliferative disorders. All patients diagnosed with CML (13 out of 50) were positive for the BCR-ABL hybrid. Six CML patients (46%) showed ABL-BCR amplifications of the Ib-BCR type. All rearrangements but one were concordant. The aberrant case presented a deletion of exon b3, in addition to the alternative Ib-BCR and Ia-BCR. Its possible origin and relevance are briefly discussed.

MeSH terms

Exons / genetics*
Fusion Proteins, bcr-abl / genetics*
Gene Deletion*
Gene Rearrangement*
Humans
Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
Prospective Studies

Substances

Fusion Proteins, bcr-abl