Quantification of Bcr-Abl transcripts in chronic myelogenous leukemia (CML) using standardized, internally controlled, competitive differential PCR (CD-PCR)

S Nagel; M Schmidt; C Thiede; D Huhn; A Neubauer

doi:10.1093/nar/24.20.4102

Quantification of Bcr-Abl transcripts in chronic myelogenous leukemia (CML) using standardized, internally controlled, competitive differential PCR (CD-PCR)

Nucleic Acids Res. 1996 Oct 15;24(20):4102-3. doi: 10.1093/nar/24.20.4102.

Authors

S Nagel¹, M Schmidt, C Thiede, D Huhn, A Neubauer

Affiliation

¹ Abteilung für Innere Medizin m.S. Hämatologie und Onkologie, Virchow Klinikum, Medizinische Fakultät der Humboldt Universität zu Berlin, Germany.

Abstract

The quantification of Bcr-Abl transcript numbers in chronic myelogenous leukemia (CML) patients described here uses simultaneous competitive PCR amplification of the target gene (Bcr-Abl) and a reference gene (porphobilinogen deaminase; Pbgd) together with a single composite competitor molecule for both targets based on heterologous sequences. Using this technique, Bcr-Abl transcript numbers could be reproducibly determined even in clinical samples known to harbour poor quality RNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA Primers / chemistry
DNA, Complementary / analysis
Electrophoresis, Agar Gel
Gene Expression Regulation, Neoplastic / genetics
Genes, abl / genetics*
Genetic Vectors / genetics
Humans
Hydroxymethylbilane Synthase / genetics
Interferons / therapeutic use
Leukemia, Myelogenous, Chronic, BCR-ABL Positive / metabolism*
Polymerase Chain Reaction / methods*
Transcription, Genetic / genetics*

Substances

DNA Primers
DNA, Complementary
Interferons
Hydroxymethylbilane Synthase