Abstract
Presenilin-2 is a gene of unknown function recently identified based upon linkage with some forms of familial Alzheimer's disease. To investigate potential effects of PS-2 on cell viability, rat pheochromocytoma (PC12) cells were stably transfected with cDNA constructs encoding either full-length human PS-2 or, for comparison, mouse Bcl-X(L). Overexpression of PS-2 conferred increased sensitivity to the apoptotic stimuli staurosporine and hydrogen peroxide. In contrast, Bcl-X(L) overexpression significantly reduced cell death induced by these stimuli. These results suggest that one function of PS-2 may involve modulation of cell viability.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Alzheimer Disease
-
Animals
-
Apoptosis*
-
Cell Survival
-
Gene Expression
-
Humans
-
Hydrogen Peroxide / pharmacology
-
Membrane Proteins / genetics
-
Membrane Proteins / physiology*
-
Mice
-
PC12 Cells
-
Presenilin-2
-
Proto-Oncogene Proteins / genetics
-
Proto-Oncogene Proteins / physiology
-
Proto-Oncogene Proteins c-bcl-2*
-
Rats
-
Staurosporine / pharmacology
-
Transfection
-
bcl-X Protein
Substances
-
BCL2L1 protein, human
-
Bcl2l1 protein, mouse
-
Bcl2l1 protein, rat
-
Membrane Proteins
-
PSEN2 protein, human
-
Presenilin-2
-
Proto-Oncogene Proteins
-
Proto-Oncogene Proteins c-bcl-2
-
bcl-X Protein
-
Hydrogen Peroxide
-
Staurosporine