Abstract
When human Bel-7402 hepatocarcinoma cell line grew in a medium containing 10(-4) M dimethyl-4,4'-dimethoxy-5,6,5', 6'-dimethylenedioxybiphynyl-2,2'-dicarboxylate (DDB), the secretion of alpha-fetoprotein (AFP) was significantly lower than the control cells, whereas the albumin (ALB) secretion was markedly higher. The activity of gamma-glutamultranspepeidase (gamma-GT) in DDB-treated cells markedly decreased in comparison with the control cells. On the contrary, the activity of tyrosine-alpha-ketoglutarate transaminase (TAT) was higher in DDB-treated cells than in control cells. DDB (10(-4) M) could significantly increase the content of cAMP in Bel-7402 cells, and also enhance the expression of anti-oncogene p53. The results suggest that DDB has reversing effects on the phenotypes of the human Bel-7402 hepatocarcinoma cell line.
MeSH terms
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Biphenyl Compounds / pharmacology*
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Carcinoma, Hepatocellular / metabolism
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Carcinoma, Hepatocellular / pathology*
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Cyclic AMP / biosynthesis
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Dioxoles / pharmacology*
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Gene Expression Regulation, Neoplastic / drug effects
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Genes, p53
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Humans
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Liver Neoplasms / metabolism
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Liver Neoplasms / pathology*
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Neoplasm Proteins / analysis*
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Neoplasm Proteins / biosynthesis
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Neoplasm Proteins / genetics
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Neoplasm Proteins / metabolism
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Phenotype
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Serum Albumin / metabolism
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Tumor Cells, Cultured / drug effects
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Tumor Suppressor Protein p53 / biosynthesis
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Tyrosine Transaminase / metabolism
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alpha-Fetoproteins / metabolism
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gamma-Glutamyltransferase / metabolism
Substances
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Biphenyl Compounds
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Dioxoles
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Neoplasm Proteins
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Serum Albumin
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Tumor Suppressor Protein p53
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alpha-Fetoproteins
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7-hydroxy-7'-methoxy-4,4'-bis(1,3-benzodioxole)-5,5'-dicarboxylic acid dimethyl ester
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Cyclic AMP
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gamma-Glutamyltransferase
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Tyrosine Transaminase