Molecular cloning and mapping of a human cDNA (SC5DL) encoding a protein homologous to fungal sterol-C5-desaturase

Cytogenet Cell Genet. 1996;74(4):252-4. doi: 10.1159/000134427.

Abstract

We have isolated a human cDNA clone homologous to fungal ERG3, a gene encoding sterol C-5 desaturase. The full nucleotide sequence of this human cDNA revealed a 708-bp open reading frame that encodes a 236-amino-acid polypeptide. The gene was expressed in all normal human tissues examined. We determined its location to chromosome 11q23.3 by fluorescence in situ hybridization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / enzymology
  • Blotting, Northern
  • Candida / enzymology
  • Chromosome Mapping*
  • Chromosomes, Human, Pair 11*
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Humans
  • In Situ Hybridization, Fluorescence
  • Molecular Sequence Data
  • Oxidoreductases / chemistry
  • Oxidoreductases / genetics*
  • Saccharomyces cerevisiae / enzymology
  • Sequence Homology, Amino Acid

Substances

  • DNA, Complementary
  • Oxidoreductases
  • sterol delta-5 desaturase

Associated data

  • GENBANK/AB016247
  • GENBANK/D85181