Abstract
We have isolated a human cDNA clone homologous to fungal ERG3, a gene encoding sterol C-5 desaturase. The full nucleotide sequence of this human cDNA revealed a 708-bp open reading frame that encodes a 236-amino-acid polypeptide. The gene was expressed in all normal human tissues examined. We determined its location to chromosome 11q23.3 by fluorescence in situ hybridization.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Arabidopsis / enzymology
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Blotting, Northern
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Candida / enzymology
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Chromosome Mapping*
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Chromosomes, Human, Pair 11*
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Cloning, Molecular
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DNA, Complementary / genetics
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Humans
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In Situ Hybridization, Fluorescence
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Molecular Sequence Data
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Oxidoreductases / chemistry
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Oxidoreductases / genetics*
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Saccharomyces cerevisiae / enzymology
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Sequence Homology, Amino Acid
Substances
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DNA, Complementary
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Oxidoreductases
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sterol delta-5 desaturase
Associated data
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GENBANK/AB016247
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GENBANK/D85181