Abstract
Primary fetal human astrocytes and an astrocytoma cell line, U373-MG, expressed membrane cofactor protein (CD46), CD59, and low levels of decay-accelerating factor (CD55). Astrocyte CD55 was capable of regulating C3 deposition on the cell surface; albeit at a lower level than primary human fibroblasts. Negligible complement-mediated lysis of primary astrocytes and the U373-MG cell line was observed, even when large amount of astrocyte-specific, complement-activating antibodies were bound to the cells. Blocking the function of CD59 on astrocytes resulted in a > 90% cell lysis, while equivalent lysis of fibroblasts could only be achieved with additional blocking of CD55.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, CD / metabolism
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Astrocytes / metabolism*
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Astrocytoma / metabolism*
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CD55 Antigens / metabolism*
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CD59 Antigens / metabolism
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Cells, Cultured
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Complement C3 / metabolism*
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Complement Inactivator Proteins / metabolism
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Fetus
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Fluoresceins / metabolism
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Fluorescent Antibody Technique, Indirect
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Gene Expression
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Glial Fibrillary Acidic Protein / metabolism
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Humans
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Membrane Cofactor Protein
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Membrane Glycoproteins / metabolism
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RNA, Messenger / genetics
Substances
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Antigens, CD
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CD46 protein, human
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CD55 Antigens
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CD59 Antigens
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Complement C3
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Complement Inactivator Proteins
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Fluoresceins
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Glial Fibrillary Acidic Protein
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Membrane Cofactor Protein
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Membrane Glycoproteins
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RNA, Messenger
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fluorexon