A genetic sequence change in the 3'-noncoding region of the androgen receptor gene in prostate carcinoma

Eur Urol. 1997;31(2):209-15. doi: 10.1159/000474452.

Abstract

Objective: We intended to identify structural changes in the hormone-binding domain and C-terminal region of the androgen receptor (AR) gene in carcinoma of prostate specimens.

Methods: Twelve prostate cancer specimens from prostate resections were analyzed for the presence of mutations in the E-H exons and part of the 3'-untranslated region of the AR cDNA, using RT-PCR and direct sequencing.

Results: A polymorphic sequence spanning 17 nucleotides was identified in the 3'-untranslated region of the AR cDNA from one prostate carcinoma tissue, suggesting the presence of a somatic mutation. No mutations were identified in the coding sequence of the hormone-binding domain of the AR cDNA.

Conclusion: These results suggest that AR mutations are probably uncommon molecular events in the early stages of prostate cancer.

MeSH terms

  • Adenocarcinoma / genetics*
  • Adenocarcinoma / pathology
  • Adenocarcinoma / surgery
  • Aged
  • Alleles
  • DNA Mutational Analysis
  • DNA Primers / chemistry
  • DNA, Complementary / analysis*
  • DNA, Neoplasm / analysis*
  • Electrophoresis, Agar Gel
  • Exons
  • Humans
  • Male
  • Middle Aged
  • Mutation / genetics*
  • Neoplasm Staging
  • Polymerase Chain Reaction
  • Prostatectomy
  • Prostatic Neoplasms / genetics*
  • Prostatic Neoplasms / pathology
  • Prostatic Neoplasms / surgery
  • RNA, Messenger / analysis
  • Receptors, Androgen / genetics*

Substances

  • DNA Primers
  • DNA, Complementary
  • DNA, Neoplasm
  • RNA, Messenger
  • Receptors, Androgen