Protein interactions regulating vesicle transport between the endoplasmic reticulum and Golgi apparatus in mammalian cells

Cell. 1997 Apr 4;89(1):149-58. doi: 10.1016/s0092-8674(00)80191-9.

Abstract

The proposed cis-Golgi vesicle receptor syntaxin 5 was found in a complex with Golgi-associated SNARE of 28 kDa (GOS-28), rbet1, rsly1, and two novel proteins characterized herein: rat sec22b and membrin, both cytoplasmically oriented integral membrane proteins. The complex appears to recapitulate vesicle docking interactions of proteins originating from distinct compartments, since syntaxin 5, rbet1, and GOS-28 localize to Golgi membranes, whereas mouse sec22b and membrin accumulate in the endoplasmic reticulum. Protein interactions in the complex are dramatically rearranged by N-ethylmaleimide-sensitive factor. The complex consists of two or more subcomplexes with some members (rat sec22b and syntaxin 5) in common and others (rbet1 and GOS-28) mutually exclusively associated. We propose that these protein interactions determine vesicle docking/fusion fidelity between the endoplasmic reticulum and Golgi.

MeSH terms

  • Animals
  • Biological Transport / physiology
  • COS Cells / physiology
  • Carrier Proteins / physiology
  • Detergents
  • Endoplasmic Reticulum / chemistry
  • Endoplasmic Reticulum / metabolism*
  • Epitopes / analysis
  • Gene Expression / physiology
  • Golgi Apparatus / chemistry
  • Golgi Apparatus / metabolism*
  • Mammals
  • Membrane Proteins / analysis
  • Membrane Proteins / genetics*
  • Membrane Proteins / isolation & purification
  • Membrane Proteins / physiology
  • Mice
  • Molecular Sequence Data
  • N-Ethylmaleimide-Sensitive Proteins
  • Nerve Tissue Proteins / analysis
  • Nerve Tissue Proteins / isolation & purification
  • Neurons / cytology
  • Neurons / metabolism
  • Qa-SNARE Proteins
  • Qb-SNARE Proteins
  • Qc-SNARE Proteins
  • R-SNARE Proteins
  • Rabbits
  • Rats
  • Rats, Sprague-Dawley
  • Sequence Homology, Amino Acid
  • Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
  • Subcellular Fractions / chemistry
  • Synaptic Vesicles / chemistry
  • Synaptic Vesicles / metabolism*
  • Vesicular Transport Proteins*

Substances

  • BET1 protein, rat
  • Carrier Proteins
  • Detergents
  • Epitopes
  • Gosr1 protein, mouse
  • Gosr1 protein, rat
  • Gosr2 protein, mouse
  • Gosr2 protein, rat
  • Membrane Proteins
  • Nerve Tissue Proteins
  • Qa-SNARE Proteins
  • Qb-SNARE Proteins
  • Qc-SNARE Proteins
  • R-SNARE Proteins
  • Sec22a protein, rat
  • Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
  • Vesicular Transport Proteins
  • N-Ethylmaleimide-Sensitive Proteins
  • Nsf protein, mouse
  • Nsf protein, rat

Associated data

  • GENBANK/U91538
  • GENBANK/U91539