Abstract
Translocations involving the human CBFA2 locus have been associated with leukemia. This gene, originally named AML1, is a human homologue of the Drosophila gene runt that controls early events in fly embryogenesis. To clarify the role of mammalian runt products in normal and leukemic hematopoiesis, we have studied their pattern of expression in mouse hematopoietic tissues in the adult and during ontogeny using an anti-runt box antiserum. In the adult bone marrow, we found expression of runt polypeptides in differentiating myeloid cells and in B lymphocytes. Within the erythroid lineage, runt expression is biphasic, clearly present in the erythroblasts of early blood islands and of the fetal liver, but absent in the adult. Biochemical analysis by Western blotting of fetal and adult hematopoietic populations shows several runt isoforms. At least one of them appears to be myeloid specific.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Age Factors
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Animals
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Blotting, Western
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Cell Lineage
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Chromosomes, Human, Pair 21 / genetics
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Chromosomes, Human, Pair 21 / ultrastructure
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Core Binding Factor Alpha 2 Subunit
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DNA-Binding Proteins / genetics
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Drosophila Proteins
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Drosophila melanogaster / embryology
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Drosophila melanogaster / genetics
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Erythroblasts / metabolism
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Fluorescent Antibody Technique, Indirect
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Gene Expression Regulation*
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Gene Expression Regulation, Leukemic
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Hematopoiesis*
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Hematopoietic System / cytology
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Hematopoietic System / embryology
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Hematopoietic System / growth & development
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Humans
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Immune Sera
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Leukemia / genetics*
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Leukemia / pathology
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Leukemia, Myeloid / genetics
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Leukemia, Myeloid / pathology
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Liver / cytology
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Liver / embryology
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Mice
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Neoplasm Proteins / biosynthesis
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Neoplasm Proteins / genetics
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Nuclear Proteins
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Organ Specificity
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Peptide Fragments / genetics
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Peptide Fragments / immunology
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Proto-Oncogene Proteins*
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Rabbits
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Recombinant Fusion Proteins / biosynthesis
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Species Specificity
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Transcription Factors / biosynthesis
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Transcription Factors / genetics*
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Translocation, Genetic
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Yolk Sac / cytology
Substances
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Core Binding Factor Alpha 2 Subunit
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DNA-Binding Proteins
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Drosophila Proteins
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Immune Sera
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Neoplasm Proteins
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Nuclear Proteins
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Peptide Fragments
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Proto-Oncogene Proteins
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RUNX1 protein, human
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Recombinant Fusion Proteins
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Runx1 protein, mouse
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Transcription Factors
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run protein, Drosophila