Abstract
Wild-type human p53 gene was transfected into the human glioma cell line T-98G. Transfectants were then isolated and characterized for growth potential and differentiation phenotype. Growth suppression, overexpression of GFAP, and accumulation in G1 phase were more commonly observed in transfectants than in T-98G cells. p21WAF1/CIP1 was overexpressed in transfectants, and the binding of PCNA and CDK 2 to p21WAF1/CIP1 were increased in transfectants. These results suggested the roles of p21WAF1/CIP1, PCNA, and CDK2 in regulation of differentiation in glioma cells and the gene transfer of wild-type p53 may be effective for the control of glial differentiation in glioma cells.
MeSH terms
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Brain Neoplasms / genetics*
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Brain Neoplasms / metabolism
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Brain Neoplasms / pathology
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CDC2-CDC28 Kinases*
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Cell Division / genetics
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Cyclin-Dependent Kinase 2
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Cyclin-Dependent Kinase Inhibitor p21
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Cyclin-Dependent Kinases / biosynthesis
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Cyclins / biosynthesis
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Gene Expression Regulation, Neoplastic
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Genes, p53 / genetics*
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Glial Fibrillary Acidic Protein / biosynthesis
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Glioma / genetics*
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Glioma / metabolism
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Glioma / pathology
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Humans
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Proliferating Cell Nuclear Antigen / biosynthesis
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Protein Serine-Threonine Kinases / biosynthesis
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Transfection
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Tumor Cells, Cultured / cytology
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Tumor Cells, Cultured / metabolism
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Tumor Stem Cell Assay
Substances
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CDKN1A protein, human
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Cyclin-Dependent Kinase Inhibitor p21
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Cyclins
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Glial Fibrillary Acidic Protein
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Proliferating Cell Nuclear Antigen
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Protein Serine-Threonine Kinases
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CDC2-CDC28 Kinases
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CDK2 protein, human
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Cyclin-Dependent Kinase 2
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Cyclin-Dependent Kinases