Abstract
Angiogenin stimulates both [3H]thymidine incorporation and proliferation of human endothelial cells in sparse cultures. Under these conditions, a 170-kDa cell surface protein can be detected that binds angiogenin specifically. Angiogenin-stimulated cell growth is concentration-dependent and is completely inhibited by an anti-angiogenin monoclonal antibody, but not by a nonimmune control antibody. It is not affected by the nonangiogenic homolog, RNase A, nor by other angiogenic proteins, such as basic fibroblast growth factor and its antibody. Results suggest that under specific conditions, endothelial cells express an angiogenin receptor that may mediate angiogenin-stimulated DNA synthesis and proliferation and play an important role in angiogenin-induced angiogenesis.
MeSH terms
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Angiogenesis Inducing Agents / pharmacology
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Antibodies, Monoclonal / pharmacology
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Cell Division / drug effects
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DNA / biosynthesis
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Endothelium, Vascular / cytology
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Endothelium, Vascular / drug effects
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Endothelium, Vascular / metabolism*
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Fibroblast Growth Factor 1 / pharmacology
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Fibroblast Growth Factor 2 / pharmacology
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Humans
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Microcirculation
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Proteins / immunology
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Proteins / pharmacology*
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Receptors, Cell Surface / isolation & purification
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Receptors, Cell Surface / physiology*
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Recombinant Proteins / pharmacology
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Ribonuclease, Pancreatic / pharmacology
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Thymidine / metabolism
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Umbilical Arteries
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Umbilical Veins
Substances
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Angiogenesis Inducing Agents
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Antibodies, Monoclonal
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Proteins
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Receptors, Cell Surface
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Recombinant Proteins
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angiogenin receptor
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Fibroblast Growth Factor 2
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Fibroblast Growth Factor 1
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DNA
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angiogenin
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Ribonuclease, Pancreatic
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Thymidine