Abstract
The islet cell antigen ICA69 is an autoimmune target in most patients with insulin-dependent diabetes. Understanding its role in diabetic autoimmunity would be facilitated by an animal model. We therefore cloned mouse ICA69. The different splice variants now identified conserve Tep69, the single T cell epitope recognized by patient T cells. We show that diabetes-prone NOD mice had Tep69-specific, autoreactive T cell repertoires and thus provide a relevant model for the study of ICA69's role in diabetic autoimmunity.
MeSH terms
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Alternative Splicing
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Amino Acid Sequence
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Animals
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Autoantigens / genetics*
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Autoantigens / immunology
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Blotting, Western
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Cloning, Molecular
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DNA, Complementary / genetics
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Diabetes Mellitus, Type 1 / immunology*
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Humans
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Membrane Proteins / genetics
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Membrane Proteins / immunology
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Mice
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Mice, Inbred NOD
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Molecular Sequence Data
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RNA, Messenger / genetics
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Sequence Alignment
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Sequence Homology, Amino Acid
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Species Specificity
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Tissue Distribution
Substances
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Autoantigens
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DNA, Complementary
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ICA1 protein, human
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Ica1 protein, mouse
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Membrane Proteins
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RNA, Messenger