Interaction of p59fyn with interferon-activated Jak kinases

Biochem Biophys Res Commun. 1997 Jun 9;235(1):83-8. doi: 10.1006/bbrc.1997.6741.

Abstract

During IFN alpha stimulation, p59(fyn) associates with the Type I IFNR-associated Tyk-2 kinase in several human hematopoietic cell lines in vivo. This interaction is direct, and is mediated by the SH2 domain in p59(fyn), as shown by binding studies using glutathione-S-transferase fusion proteins and far western blots. Furthermore, in response to IFN alpha-treatment of cells, the SH2 domain of Fyn interacts with the Tyk-2-associated c-cbl proto-oncogene product. In a similar manner, during IFN gamma stimulation, p59(fyn) associates via its SH2 domain with the activated form of the IFN gamma-dependent Jak-2 kinase. These data suggest that p59(fyn) is a common element in IFN alpha and IFN gamma signaling, and is selectively engaged by the Type I or II IFN receptors via specific interactions with distinct Jak kinases.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / metabolism
  • Blotting, Western
  • Glutathione Transferase / genetics
  • Humans
  • Interferon Type I / pharmacology*
  • Interferon-gamma / pharmacology
  • Janus Kinase 1
  • Janus Kinase 2
  • Phosphotyrosine / immunology
  • Protein-Tyrosine Kinases / metabolism*
  • Proteins / metabolism
  • Proto-Oncogene Mas
  • Proto-Oncogene Proteins / immunology
  • Proto-Oncogene Proteins / metabolism*
  • Proto-Oncogene Proteins c-cbl
  • Proto-Oncogene Proteins c-fyn
  • Recombinant Fusion Proteins / metabolism
  • Recombinant Proteins
  • Signal Transduction / physiology
  • TYK2 Kinase
  • Tumor Cells, Cultured
  • Ubiquitin-Protein Ligases*
  • src Homology Domains

Substances

  • Antibodies, Monoclonal
  • Interferon Type I
  • MAS1 protein, human
  • Proteins
  • Proto-Oncogene Mas
  • Proto-Oncogene Proteins
  • Recombinant Fusion Proteins
  • Recombinant Proteins
  • Phosphotyrosine
  • Interferon-gamma
  • Proto-Oncogene Proteins c-cbl
  • Ubiquitin-Protein Ligases
  • Glutathione Transferase
  • Protein-Tyrosine Kinases
  • FYN protein, human
  • JAK1 protein, human
  • JAK2 protein, human
  • Janus Kinase 1
  • Janus Kinase 2
  • Proto-Oncogene Proteins c-fyn
  • TYK2 Kinase
  • TYK2 protein, human
  • CBL protein, human