Abstract
Full length cDNAs for p53 were made by reverse transcription-polymerase chain reaction of total RNA from two normal woodchuck livers. Two randomly chosen clones from each liver were sequenced and shown to be identical. This sequence revealed 80% or more identity with p53 sequences from human, monkey, and mouse. The cDNA was translated into a 55 kD protein in vitro that was immunoprecipitated by antibodies to p53. Cotranslation of woodchuck p53 with woodchuck hepatitis virus X antigen, followed by immunoprecipitation suggested X/p53 complex formation. Similar complexes were also immunoprecipitated from extracts of infected liver, but not from uninfected liver. The finding of X/p53 complexes in vivo and in vitro in the woodchuck hepadnavirus system, combined with analogous data with hepatitis B, suggests a common mechanism by which these viruses contribute to hepatocellular transformation.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Consensus Sequence
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Genes, p53*
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Haplorhini
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Hepatitis B Virus, Woodchuck / genetics*
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Humans
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Liver / metabolism
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Liver / virology
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Liver Neoplasms / genetics*
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Liver Neoplasms / veterinary*
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Liver Neoplasms / virology
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Marmota
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Mice
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Molecular Sequence Data
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Polymerase Chain Reaction
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Protein Biosynthesis
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Protein Structure, Secondary
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Sequence Alignment
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Sequence Homology, Amino Acid
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Trans-Activators / biosynthesis*
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Trans-Activators / chemistry
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Trans-Activators / isolation & purification
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Tumor Suppressor Protein p53 / biosynthesis*
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Tumor Suppressor Protein p53 / chemistry
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Tumor Suppressor Protein p53 / isolation & purification
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Viral Regulatory and Accessory Proteins
Substances
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Trans-Activators
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Tumor Suppressor Protein p53
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Viral Regulatory and Accessory Proteins
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hepatitis B virus X protein