Subtyping of human immunodeficiency virus type 1 strains by using antibodies specific for the third variable domain (V3) of gp120: results may be affected by divergent V3 sequences

K Sherefa; M Sällberg; B Johansson; M Salminen; A Sönnerborg

doi:10.1128/jcm.35.9.2419-2421.1997

Subtyping of human immunodeficiency virus type 1 strains by using antibodies specific for the third variable domain (V3) of gp120: results may be affected by divergent V3 sequences

J Clin Microbiol. 1997 Sep;35(9):2419-21. doi: 10.1128/jcm.35.9.2419-2421.1997.

Authors

K Sherefa¹, M Sällberg, B Johansson, M Salminen, A Sönnerborg

Affiliation

¹ Department of Immunology, Microbiology, Pathology, and Infectious Diseases, Karolinska Institutet, Huddinge Hospital, Sweden.

Abstract

Human immunodeficiency virus type 1 serotype C was found in 545 of 712 Ethiopian patients by peptide enzyme immunoassay. Serotyping failed in 146 samples due to the absence of V3 antibodies or multiple reactivities. In 6 of 34 samples, discordant results were obtained by serotyping and genotyping, possibly due to divergent V3 sequences.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Epitopes / immunology
Ethiopia / epidemiology
HIV Antibodies / immunology*
HIV Envelope Protein gp120 / chemistry
HIV Envelope Protein gp120 / immunology*
HIV Infections / epidemiology
HIV Infections / immunology
HIV-1 / classification
HIV-1 / genetics
HIV-1 / immunology*
Humans
Immunoenzyme Techniques
Molecular Sequence Data
Peptides / chemical synthesis
Peptides / immunology
Phylogeny
Serotyping*

Substances

Epitopes
HIV Antibodies
HIV Envelope Protein gp120
Peptides