The detection of micrometastases in the peripheral blood and bone marrow of patients with breast cancer using immunohistochemistry and reverse transcriptase polymerase chain reaction for keratin 19

Eur J Cancer. 1997 May;33(6):854-61. doi: 10.1016/s0959-8049(97)00014-2.

Abstract

The aim of this study was to determine whether reverse transcriptase polymerase chain reaction (RT-PCR) for keratin 19 (K19) provides additional information when combined with immunohistochemistry when used to detect micrometastases in blood and bone marrow in patients with primary breast cancer. We studied 78 patients with breast cancer who had no evidence of distant metastases. We collected blood and bone marrow, separated the mononuclear fraction and carried out RT-PCR and immunohistochemistry for K19. RT-PCR was done by two 40-cycle rounds using nested primers. In initial experiments, RT-PCR was shown to be capable of detecting one tumour cell in one million normal bone marrow cells, which was at least 10 times more sensitive than immunohistochemistry, while retaining specificity. Five per cent of the peripheral blood and 22% of the bone marrow samples contained K19 positive cells by immunohistochemistry staining. Using RT-PCR, these proportions increased to 25% and 35%, respectively. This represents a significantly greater detection frequency (P < 0.001 and P = 0.03, respectively). RT-PCR for K19 is a more sensitive method for detecting micrometastases in patients with primary breast cancer when compared with immunohistochemistry.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Antibodies, Monoclonal
  • Biomarkers, Tumor / analysis*
  • Biomarkers, Tumor / blood
  • Bone Marrow / chemistry*
  • Bone Marrow Neoplasms / chemistry
  • Bone Marrow Neoplasms / pathology
  • Bone Marrow Neoplasms / secondary*
  • Breast Neoplasms / blood
  • Breast Neoplasms / pathology*
  • Female
  • Humans
  • Immunohistochemistry
  • Keratins / analysis*
  • Keratins / genetics
  • Keratins / immunology
  • Middle Aged
  • Neoplasm Metastasis / diagnosis*
  • Neoplastic Cells, Circulating / chemistry*
  • Neoplastic Cells, Circulating / pathology
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis
  • Sensitivity and Specificity

Substances

  • Antibodies, Monoclonal
  • Biomarkers, Tumor
  • RNA, Messenger
  • Keratins