Abstract
cDNAs encoding a novel DNA binding protein, termed Finb (finger protein in nuclear bodies) were molecularly cloned. The Finb protein consists of 1656 amino acids, containing 15 C2H2-type zinc fingers and three proline-rich regions. Finb efficiently activates transcription from the promoters of the metallothionein and thymidine kinase genes. The finb mRNA is ubiquitously expressed in various cell lines and tissues. The protein product is localized in nuclear bodies, whereas the N-terminally truncated protein spreads throughout the nucleus and cytoplasm. Further characterization of Finb would unravel an important role of nuclear body-involved transcriptional regulation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Blotting, Western
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COS Cells
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Cell Nucleus / genetics
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Cell Nucleus / metabolism
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Cells, Cultured
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Chloramphenicol O-Acetyltransferase / metabolism
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Chlorocebus aethiops
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Cloning, Molecular
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Cytoplasm / metabolism
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Epidermal Growth Factor / metabolism
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Fluorescent Antibody Technique, Indirect
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Gene Expression
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Genes, erbB-2
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Humans
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Metallothionein / genetics
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Molecular Sequence Data
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Plasmids
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Promoter Regions, Genetic
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RNA, Messenger / metabolism
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Sequence Alignment
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Thymidine Kinase / genetics
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Trans-Activators / genetics*
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Trans-Activators / metabolism
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Transcription, Genetic
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Transcriptional Activation
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Zinc Fingers / genetics*
Substances
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RNA, Messenger
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Trans-Activators
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Epidermal Growth Factor
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Metallothionein
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Chloramphenicol O-Acetyltransferase
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Thymidine Kinase