Abstract
TRAIL-R3, a new member of the TRAIL receptor family, has been cloned and characterized. TRAIL-R3 encodes a 299 amino acid protein with 58 and 54% overall identity to TRAIL-R1 and -R2, respectively. Transient expression and quantitative binding studies show TRAIL-R3 to be a plasma membrane-bound protein capable of high affinity interaction with the TRAIL ligand. The TRAIL-R3 gene maps to human chromosome 8p22-21, clustered with the genes encoding two other TRAIL receptors. In contrast to TRAIL-R1 and -R2, this receptor shows restricted expression, with transcripts detectable only in peripheral blood lymphocytes and spleen. The structure of TRAIL-R3 is unique when compared to the other TRAIL receptors in that it lacks a cytoplasmic domain and appears to be glycosyl-phosphatidylinositol-linked. Moreover, unlike TRAIL-R1 and -R2, in a transient overexpression system TRAIL-R3 does not induce apoptosis.
MeSH terms
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Amino Acid Sequence
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Apoptosis / physiology
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Apoptosis Regulatory Proteins
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Base Sequence
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Blotting, Northern
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Chromosome Mapping
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Chromosomes, Human, Pair 8 / genetics
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Cloning, Molecular
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GPI-Linked Proteins
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Gene Expression Regulation
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Glycosylphosphatidylinositols
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Humans
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Immunoglobulin Fc Fragments / genetics
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Jurkat Cells
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Lymphocytes / chemistry
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Lymphocytes / metabolism
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Membrane Glycoproteins / metabolism*
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Membrane Glycoproteins / pharmacology
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Molecular Sequence Data
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Protein Binding
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Receptors, Cell Surface / chemistry*
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / metabolism*
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Receptors, Tumor Necrosis Factor*
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Receptors, Tumor Necrosis Factor, Member 10c
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Recombinant Fusion Proteins / pharmacology
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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TNF-Related Apoptosis-Inducing Ligand
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Tumor Necrosis Factor Decoy Receptors
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Tumor Necrosis Factor-alpha / metabolism*
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Tumor Necrosis Factor-alpha / pharmacology
Substances
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Apoptosis Regulatory Proteins
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GPI-Linked Proteins
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Glycosylphosphatidylinositols
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Immunoglobulin Fc Fragments
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Membrane Glycoproteins
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Receptors, Cell Surface
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Receptors, Tumor Necrosis Factor
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Receptors, Tumor Necrosis Factor, Member 10c
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Recombinant Fusion Proteins
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TNF-Related Apoptosis-Inducing Ligand
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TNFRSF10C protein, human
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TNFSF10 protein, human
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Tumor Necrosis Factor Decoy Receptors
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Tumor Necrosis Factor-alpha