Abstract
Since GH stimulates the development and function of granulocytes, we investigated the expression of GH in granulocyte subsets. By immunocytochemistry, 25 +/- 7% of the human neutrophils were shown to express immunoreactive GH, whereas eosinophils were negative. Reversed transcription (RT)-PCR analysis demonstrated GH mRNA in neutrophils. Restriction analysis revealed that neutrophils express the GH-N gene but not the GH-V gene. Furthermore, we demonstrated by western blot analysis that neutrophils express an alternatively spliced variant of the pituitary transcription factor Pit-1, designated Pit-1b.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adenoma / chemistry
-
Adenoma / pathology
-
Adult
-
Blotting, Western
-
DNA-Binding Proteins / biosynthesis*
-
DNA-Binding Proteins / genetics*
-
DNA-Binding Proteins / physiology
-
Gene Expression Regulation
-
Genetic Variation
-
Granulocytes / chemistry
-
Granulocytes / cytology
-
Granulocytes / metabolism
-
Growth Hormone / biosynthesis*
-
Growth Hormone / genetics*
-
Growth Hormone / physiology
-
Humans
-
Immunohistochemistry
-
Middle Aged
-
Neutrophils / chemistry
-
Neutrophils / cytology
-
Neutrophils / metabolism*
-
Pituitary Gland / chemistry*
-
Pituitary Gland / cytology
-
Pituitary Neoplasms / chemistry
-
Pituitary Neoplasms / pathology
-
Polymerase Chain Reaction
-
RNA, Messenger / analysis
-
RNA, Messenger / genetics
-
RNA, Messenger / metabolism
-
Transcription Factor Pit-1
-
Transcription Factors / biosynthesis*
-
Transcription Factors / genetics*
-
Transcription Factors / physiology
-
Transcription, Genetic*
Substances
-
DNA-Binding Proteins
-
POU1F1 protein, human
-
RNA, Messenger
-
Transcription Factor Pit-1
-
Transcription Factors
-
Growth Hormone