Lipopolysaccharides of Helicobacter pylori serogroups O:3 and O:6--structures of a class of lipopolysaccharides with reference to the location of oligomeric units of D-glycero-alpha-D-manno-heptose residues

Eur J Biochem. 1997 Sep 1;248(2):592-601. doi: 10.1111/j.1432-1033.1997.00592.x.

Abstract

Lipopolysaccharides (LPS) from antigenically different strains assigned to serogroups O:3 and O:6 of Helicobacter pylori were isolated as water-soluble material of high Mr and as water-insoluble gels of low Mr. Chemical and spectroscopic analyses of the soluble LPS and oligosaccharides liberated from the water-insoluble gels led to proposed structures with Lewis (Le) antigen determinants terminating regular repeating units of different types, linked in turn to inner core regions of invariable structure. The O:6 LPS has two populations of related molecules with chains of 3-linked D-glycero-alpha-D-manno-heptose residues similar to those in the MO19 strain, one with and the other without a single terminal Lewis (Le(y)) epitope. In contrast, in the O:3 LPS, Lewis (Le(x) and Le(y)) epitopes terminate a partially fucosylated N-acetyllactosaminoglycan, but a heptan chain similar to that in the O:6 LPS was shown to connect the outer chains to the inner core. These LPS provide examples of the molecular mimicry of cell-surface glycoconjugates. Structural variations of LPS between strains, and differences in some aspects of structure within strains, between high Mr and low Mr LPS indicate a class of LPS whose mechanisms of biosynthesis lead to overall architectures different from those characteristic of most LPS from enteric bacteria.

MeSH terms

  • Amino Sugars / chemistry
  • Amino Sugars / metabolism
  • Carbohydrate Sequence
  • Carbohydrates / analysis
  • Helicobacter pylori / chemistry*
  • Helicobacter pylori / immunology
  • Lewis Blood Group Antigens / chemistry
  • Lipopolysaccharides / chemistry*
  • Lipopolysaccharides / immunology*
  • Lipopolysaccharides / metabolism
  • Molecular Sequence Data
  • O Antigens / chemistry
  • Polysaccharides / chemistry
  • Polysaccharides / metabolism
  • Solubility

Substances

  • Amino Sugars
  • Carbohydrates
  • Lewis Blood Group Antigens
  • Lewis Y antigen
  • Lipopolysaccharides
  • O Antigens
  • Polysaccharides
  • lactosaminoglycan