Abstract
We characterized the gntT gene encoding a high-affinity gluconate permease of Escherichia coli K-12. Primer extension and lacZ-operon fusion analyses revealed that gntT has one strong and two weak promoters, all of which are regulated positively by cAMP-CRP and negatively by GntR. The weak promoters became constitutive when separated from the upstream region including the strong promoter that overlaps a putative GntR-binding sequence. Gluconate-specific uptake activity was observed with cells harboring the gntT plasmid clone, which was enhanced by the presence of gntK encoding gluconate kinase.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Bacterial Proteins / genetics*
-
Bacterial Proteins / metabolism*
-
Base Sequence
-
Biological Transport
-
Carbohydrates
-
Carrier Proteins
-
Cyclic AMP / pharmacology
-
Cyclic AMP Receptor Protein / metabolism
-
Escherichia coli / enzymology
-
Escherichia coli / genetics*
-
Escherichia coli Proteins*
-
Gene Expression Regulation, Bacterial / drug effects
-
Gene Expression Regulation, Bacterial / physiology*
-
Genes, Bacterial / physiology
-
Gluconates / metabolism
-
Gluconates / pharmacology
-
Kinetics
-
Membrane Transport Proteins / genetics*
-
Molecular Sequence Data
-
Operator Regions, Genetic / genetics
-
Phosphotransferases (Alcohol Group Acceptor) / physiology
-
Promoter Regions, Genetic / genetics
-
Sequence Analysis, DNA
Substances
-
Bacterial Proteins
-
Carbohydrates
-
Carrier Proteins
-
Cyclic AMP Receptor Protein
-
Escherichia coli Proteins
-
Gluconates
-
Membrane Transport Proteins
-
gluconate permease, E coli
-
Cyclic AMP
-
Phosphotransferases (Alcohol Group Acceptor)
-
gluconokinase
-
gluconic acid