Molecular cloning and characterization of P113, a mouse SNF2/SWI2-related transcription factor

Gene. 1997 Nov 20;202(1-2):31-7. doi: 10.1016/s0378-1119(97)00446-0.

Abstract

A full-length cDNA encoding a 113-kDa transcription factor, named P113, was cloned from mouse preadipocyte line 30A5. P113 binds to a 7-bp consensus TNF-response element and a 30-bp fragment from mouse PAI-1 promoter (-88/-59). Sequence analysis indicates that the P113 is highly homologous to HIP116/HLTF (human) and RUSH-1alpha (rabbit). The sequence homology and the fact that P113 contains seven motifs conserved in many DNA-dependent helicases/ATPases indicate that it is a new member of the SNF2/SWI2 protein family. A cysteine-rich motif, called RING finger, was found close to the C-terminus of P113. The expression pattern of P113 mRNA in rat tissues is significantly different from that of HLTF in human tissues. Affinity-purified P113 has an ATPase activity that is activated by DNA in a sequence-specific manner. Using Northern blot analysis and the PAI-1 promoter/luciferase system, we demonstrated that P113 is a transcription factor that activates the transcription of the PAI-1 gene in 30A5 cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphatases / metabolism
  • Adipocytes
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • DNA Helicases*
  • DNA-Binding Proteins / biosynthesis
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / isolation & purification*
  • DNA-Binding Proteins / metabolism
  • DNA-Binding Proteins / physiology
  • Gene Expression Regulation / drug effects
  • HeLa Cells
  • Humans
  • Mice
  • Molecular Sequence Data
  • Nuclear Proteins*
  • Organ Specificity / genetics
  • Plasminogen Activator Inhibitor 1 / genetics
  • RNA, Messenger / metabolism
  • Rabbits
  • Rats
  • Sequence Homology, Amino Acid
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics*
  • Transcription Factors / isolation & purification*
  • Transcription Factors / physiology

Substances

  • DNA-Binding Proteins
  • Nuclear Proteins
  • Plasminogen Activator Inhibitor 1
  • RNA, Messenger
  • SMARCA2 protein, human
  • Smarca2 protein, mouse
  • Smarca3 protein, mouse
  • Transcription Factors
  • Adenosine Triphosphatases
  • SMARCA4 protein, human
  • Smarca4 protein, mouse
  • DNA Helicases