Changes in enamel during its development are permanently recorded, and commonly present as either demarcated opacity, diffuse opacity, or enamel hypoplasia. Developmental enamel defects may provide clues regarding their aetiology, and this may have application in clinical dentistry, dental epidemiology and anthropology. However, the usefulness of these applications may be hampered by many pitfalls encountered in the detection and diagnosis of developmental enamel defects. The defects may be masked by saliva, dental plaque, and incorrect lighting. In addition, confounding effects of post-eruptive changes such as dental caries, attrition, and traumatic loss of tooth structure may impair the detection of developmental enamel defects. The non-specificity of appearance of enamel defects may make aetiologic diagnosis of enamel hypoplasia difficult. Furthermore, difficulty is often encountered in the relative timing of events in enamel hypoplasia, due to limited data on the chronology of development of the human dentition. In this review, pitfalls in the clinical assessment of enamel defects are discussed, and guidelines to overcome some of these difficulties are presented.