Abstract
We examined the expression levels of P-glycoprotein (P-Gp)/the human multidrug resistance gene (MDR1) and in vivo chemosensitivity in the 7 osteosarcoma xenografts. Three of seven (43%) osteosarcoma xenografts expressed MDR1 by reverse transcriptase-polymerase chain reaction (RT-PCR). The OSS-516R xenograft selected with vincristine (VCR) from the MDR1-negative xenograft OSS-516, which was sensitive to VCR and doxorubicin (DOX), acquired cross-resistance to DOX. In the OSS-516R, RT-PCR assay showed definite MDR1 expression and immunohistochemical analysis demonstrated P-Gp-positive tumor cells. These results suggest that P-Gp/MDR1 overexpression is related to multidrug resistance in human osteosarcoma in vivo.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism*
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Animals
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Bone Neoplasms / drug therapy
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Bone Neoplasms / genetics
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Bone Neoplasms / metabolism*
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Doxorubicin / therapeutic use
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Drug Resistance, Multiple / genetics*
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Gene Expression
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Genes, MDR
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Humans
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Immunohistochemistry
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Mice
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Mice, Nude
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Molecular Sequence Data
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Osteosarcoma / drug therapy
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Osteosarcoma / genetics
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Osteosarcoma / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction
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Vincristine / therapeutic use
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Xenograft Model Antitumor Assays
Substances
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Vincristine
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Doxorubicin