Abstract
Tcf transcription factors interact with beta-catenin and Armadillo to mediate Wnt/Wingless signaling. We now report the characterization of genes encoding two murine members of the Tcf family, mTcf-3 and mTcf-4. mTcf-3 mRNA is ubiquitously present in embryonic day 6.5 (E6.5) mouse embryos but gradually disappears over the next 3 to 4 days. mTcf-4 expression occurs first at E10.5 and is restricted to di- and mesencephalon and the intestinal epithelium during embryogenesis. The mTcf-3 and mTcf-4 proteins bind a canonical Tcf DNA motif and can complex with the transcriptional coactivator beta-catenin. Overexpression of Wnt-1 in a mammary epithelial cell line leads to the formation of a nuclear complex between beta-catenin and Tcf proteins and to Tcf reporter gene transcription. These data demonstrate a direct link between Wnt stimulation and beta-catenin/Tcf transcriptional activation and imply a role for mTcf-3 and -4 in early Wnt-driven developmental decisions in the mouse embryo.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Amino Acid Sequence
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Animals
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Cell Line
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Cell Nucleus / metabolism
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Chickens
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Cloning, Molecular
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Cytoskeletal Proteins / metabolism*
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Embryonic and Fetal Development
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Gene Expression
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Genes, Reporter
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HMGB Proteins*
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Humans
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Luciferases / genetics
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Mice
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Molecular Sequence Data
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PC12 Cells
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Proto-Oncogene Proteins / biosynthesis
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins / metabolism*
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Rats
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Signal Transduction*
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TCF Transcription Factors
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Trans-Activators*
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Transcription Factor 7-Like 1 Protein
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Transcription Factor 7-Like 2 Protein
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Transcription Factors / biosynthesis
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Transcription Factors / metabolism*
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Transcriptional Activation
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Wnt Proteins
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Wnt1 Protein
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Zebrafish Proteins*
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beta Catenin
Substances
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CTNNB1 protein, human
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CTNNB1 protein, mouse
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Ctnnb1 protein, rat
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Cytoskeletal Proteins
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HMGB Proteins
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Proto-Oncogene Proteins
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TCF Transcription Factors
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TCF7L1 protein, human
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TCF7L2 protein, human
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Tcf7l1 protein, mouse
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Tcf7l1 protein, rat
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Tcf7l2 protein, mouse
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Tcf7l2 protein, rat
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Trans-Activators
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Transcription Factor 7-Like 1 Protein
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Transcription Factor 7-Like 2 Protein
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Transcription Factors
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WNT1 protein, human
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Wnt Proteins
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Wnt1 Protein
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Wnt1 protein, mouse
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Wnt1 protein, rat
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Zebrafish Proteins
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beta Catenin
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Luciferases
Associated data
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GENBANK/AJ223069
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GENBANK/AJ223070