Abstract
MDA-468 human breast cancer cells overexpress the EGFR and exhibit a functional TGFalpha-EGFR autocrine pathway. Loss of EGFR expression following stable transfection with an antisense EGFR cDNA containing plasmid down-regulates type I cAMP-dependent protein kinase (PKAI) expression with acquisition of cell growth resistance to the PKAI inhibitor 8-Cl-cAMP. These results suggest that PKAI expression and function are controlled by a TGFalpha-EGFR autocrine pathway in human breast cancer cells overexpressing the EGFR.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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8-Bromo Cyclic Adenosine Monophosphate / analogs & derivatives
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8-Bromo Cyclic Adenosine Monophosphate / pharmacology
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Breast Neoplasms / enzymology*
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Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors*
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Cyclic AMP-Dependent Protein Kinases / genetics
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DNA, Antisense / pharmacology*
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Down-Regulation
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ErbB Receptors / genetics
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ErbB Receptors / physiology*
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Female
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Humans
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Transfection
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Transforming Growth Factor alpha / physiology
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Tumor Cells, Cultured
Substances
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DNA, Antisense
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Transforming Growth Factor alpha
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8-Bromo Cyclic Adenosine Monophosphate
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8-chloro-cyclic adenosine monophosphate
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ErbB Receptors
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Cyclic AMP-Dependent Protein Kinases