Abstract
This study examined the effect of n-6 polyunsaturated fatty acids (PUFAs) on the expression of nm-23, a metastasis-suppressor gene, in two highly invasive human cancer cell lines, HT115 and MDA MB 231. A range of n-6 and n-3 PUFAs were tested. We report that while linoleic acid and arachidonic acid reduced the expression of nm-23-H1, gamma linolenic acid (GLA) and its soluble lithium salt markedly increased the expression of the molecules. The stimulation of the expression of nm-23 by GLA was seen at both protein and mRNA levels. Up-regulation of nm-23 was also associated with a reduction of the in vitro invasiveness of these cells. It is concluded that gamma linolenic acid (GLA) enhances the expression of nm-23. This contributes to the inhibition of the in vitro invasion of tumour cells.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Arachidonic Acid / pharmacology
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Breast Neoplasms / genetics*
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Breast Neoplasms / pathology
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Colonic Neoplasms / genetics*
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Colonic Neoplasms / pathology
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Drug Screening Assays, Antitumor
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Eicosapentaenoic Acid / pharmacology
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Gene Expression Regulation, Neoplastic / drug effects*
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Humans
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Linoleic Acid / pharmacology*
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Monomeric GTP-Binding Proteins*
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NM23 Nucleoside Diphosphate Kinases
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Neoplasm Invasiveness
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Neoplasm Proteins / biosynthesis*
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Neoplasm Proteins / genetics
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Nucleoside-Diphosphate Kinase*
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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RNA, Neoplasm / biosynthesis
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RNA, Neoplasm / genetics
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Transcription Factors / biosynthesis*
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Transcription Factors / genetics
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Tumor Cells, Cultured
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gamma-Linolenic Acid / pharmacology*
Substances
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NM23 Nucleoside Diphosphate Kinases
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Neoplasm Proteins
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RNA, Messenger
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RNA, Neoplasm
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Transcription Factors
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Arachidonic Acid
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gamma-Linolenic Acid
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Linoleic Acid
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Eicosapentaenoic Acid
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Nucleoside-Diphosphate Kinase
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Monomeric GTP-Binding Proteins