A germline mutation abolishing the original stop codon of the human adenine phosphoribosyltransferase (APRT) gene leads to complete loss of the enzyme protein

Hum Genet. 1998 Feb;102(2):197-202. doi: 10.1007/s004390050677.

Abstract

Adenine phosphoribosyltransferase (APRT) is a purine metabolic enzyme and a homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Various germline abnormalities have been described, but we report here a unique type of germline mutation in a homozygous individual (SY) who had excreted 2,8-dihydroxyadenine crystals. In SY, TCA was substituted for the physiological stop codon TGA. This base substitution generates a new HinfI restriction site, and, using the polymerase chain reaction and subsequent digestion by this enzyme, it was confirmed that SY is homozygous for the base substitution. This base change is unique in that it generates an open reading frame that extends to the poly(A) addition site. The amount of mRNA in transformed B cells from SY was approximately a quarter of that in control subjects and no APRT proteins were detected. In eukaryotes, unlike in prokaryotes, no rescue systems for defective polypeptide termination caused by a missing stop codon have been found. Therefore, the outcome of the defect of SY is unclear from present knowledge about termination of polypeptide synthesis. Investigations into the mechanisms of the absence of protein in the cells of SY may lead to a better understanding of the physiological and nonphysiological termination of polypeptide synthesis in eukaryotic cells.

Publication types

  • Case Reports
  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenine Phosphoribosyltransferase / deficiency*
  • Adenine Phosphoribosyltransferase / genetics*
  • Adenine Phosphoribosyltransferase / isolation & purification
  • Adult
  • B-Lymphocytes
  • Blotting, Western
  • Cell Line, Transformed
  • Codon, Terminator / genetics*
  • Deoxyribonucleases, Type II Site-Specific / metabolism
  • Germ-Line Mutation*
  • Homozygote
  • Humans
  • Male
  • Point Mutation
  • Polymorphism, Single-Stranded Conformational
  • RNA, Messenger / analysis
  • Restriction Mapping
  • Sequence Analysis, DNA
  • Urinary Calculi / enzymology
  • Urinary Calculi / genetics

Substances

  • Codon, Terminator
  • RNA, Messenger
  • Adenine Phosphoribosyltransferase
  • Deoxyribonucleases, Type II Site-Specific
  • GANTC-specific type II deoxyribonucleases