Substance P induces secretion of immunomodulatory cytokines by human astrocytoma cells

J Neuroimmunol. 1998 Jan;81(1-2):127-37. doi: 10.1016/s0165-5728(97)00167-7.

Abstract

In human astrocytoma cell lines, substance P (SP) stimulated interleukin (IL)-8, IL-6, granulocyte macrophage colony-stimulating factor and leukemia inhibitory factor protein secretion. These SP effects were blocked by a specific NK1 tachykinin receptor antagonist. Further, SP stimulation increased the half-life of IL-6 and IL-8 messenger RNAs, suggesting that the synthesis of these cytokines is also regulated post-transcriptionally. SP-induced cytokine release was inhibited by staurosporine and phorbol 12-myristate 13-acetate desensitization suggesting protein kinase C involvement. The demonstration that SP affects cytokine production in glioma cells might be of relevance for the biology of such tumors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Astrocytoma / genetics
  • Astrocytoma / metabolism
  • Astrocytoma / pathology*
  • Brain Neoplasms / genetics
  • Brain Neoplasms / metabolism
  • Brain Neoplasms / pathology*
  • Cytokines / metabolism*
  • Dipeptides / pharmacology
  • Enzyme Inhibitors / pharmacology
  • Gene Expression Regulation, Neoplastic / drug effects
  • Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
  • Growth Inhibitors / metabolism
  • Humans
  • Indoles / pharmacology
  • Interleukin-6 / biosynthesis
  • Interleukin-6 / genetics
  • Interleukin-6 / metabolism
  • Interleukin-8 / biosynthesis
  • Interleukin-8 / genetics
  • Interleukin-8 / metabolism
  • Leukemia Inhibitory Factor
  • Lymphokines / metabolism
  • Neoplasm Proteins / antagonists & inhibitors
  • Neoplasm Proteins / physiology*
  • Neurokinin-1 Receptor Antagonists
  • Protein Kinase C / antagonists & inhibitors
  • Protein Kinase C / metabolism
  • RNA, Messenger / metabolism
  • RNA, Neoplasm / metabolism
  • Receptors, Neurokinin-1 / physiology*
  • Staurosporine / pharmacology
  • Substance P / pharmacology*
  • Tetradecanoylphorbol Acetate / pharmacology
  • Tumor Cells, Cultured

Substances

  • Cytokines
  • Dipeptides
  • Enzyme Inhibitors
  • Growth Inhibitors
  • Indoles
  • Interleukin-6
  • Interleukin-8
  • LIF protein, human
  • Leukemia Inhibitory Factor
  • Lymphokines
  • Neoplasm Proteins
  • Neurokinin-1 Receptor Antagonists
  • RNA, Messenger
  • RNA, Neoplasm
  • Receptors, Neurokinin-1
  • FK 888
  • Substance P
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Protein Kinase C
  • Staurosporine
  • Tetradecanoylphorbol Acetate