A panel set for epitope analysis of myeloperoxidase (MPO)-specific antineutrophil cytoplasmic antibody MPO-ANCA using recombinant hexamer histidine-tagged MPO deletion mutants

J Clin Immunol. 1998 Mar;18(2):142-52. doi: 10.1023/a:1023251001261.

Abstract

A major target protein of antineutrophil cytoplasmic antibody with a perinuclear staining pattern (P-ANCA) has been identified as myeloperoxidase (MPO). Recombinant deletion mutants of MPO, eight fragments of the heavy-chain subunit, and two fragments of the light chain subunit were expressed in E. coli using a pQE expression vector. The recombinant hexamer histidine-tagged fragments were partially purified as the denatured proteins on a Ni2+-charged nitrirotriacetic acid column. The recombinant fragments were reacted with a rabbit polyclonal antibody to human MPO in Western blotting. In addition, the reactivities of the proteins with MPO-ANCA-positive sera of four patients with renal diseases were examined by Western blotting. The profile of the reactivity showed that different sera recognized different sets of fragments of the heavy chain, whereas no serum reacted with the fragments of the light chain. These results indicate that the sera of patients with MPO-ANCA-positive diseases showed varied reactivities with the different fragments. Furthermore, an ELISA system using a set of the fragments completely purified by Sephacryl S-200HR column chromatography was established. The panel set is useful for subclassification of MPO-ANCA-related diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies
  • Antibodies, Antineutrophil Cytoplasmic / immunology*
  • Antibody Specificity
  • Chromatography / methods
  • Enzyme-Linked Immunosorbent Assay
  • Epitope Mapping / methods*
  • Epitopes / analysis*
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Histidine / analysis*
  • Humans
  • Kidney Diseases / blood
  • Kidney Diseases / enzymology
  • Kidney Diseases / immunology
  • Mutation
  • Nitrilotriacetic Acid / analogs & derivatives
  • Organometallic Compounds
  • Peptide Fragments / immunology
  • Peptide Fragments / isolation & purification
  • Peroxidase / immunology*
  • Rabbits
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / immunology
  • Recombinant Proteins / isolation & purification

Substances

  • Antibodies
  • Antibodies, Antineutrophil Cytoplasmic
  • Epitopes
  • Organometallic Compounds
  • Peptide Fragments
  • Recombinant Proteins
  • nickel nitrilotriacetic acid
  • Histidine
  • Peroxidase
  • Nitrilotriacetic Acid