Abstract
A human T-acute lymphoblastic leukemia (ALL) cell line (Loucy), derived from cells from a patient with resistant ALL with a t(16:20) and 5q- chromosomal aberrations was evaluated for p53 gene alterations and expression. Western blot analysis of p53 showed elevated levels of the protein. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis and direct sequencing identified a point mutation at codon 272 (GTG --> ATG) of the p53 gene. Possible molecular mechanisms underlying these alterations and their role in the establishment of this cell line and in leukemogenesis in general are discussed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Blotting, Western
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Chromosome Aberrations*
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Chromosomes, Human, Pair 16 / genetics*
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Chromosomes, Human, Pair 16 / ultrastructure
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Chromosomes, Human, Pair 20 / genetics*
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Chromosomes, Human, Pair 20 / ultrastructure
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Chromosomes, Human, Pair 5 / ultrastructure*
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Codon / genetics
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Drug Resistance, Neoplasm
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Female
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Genes, p53*
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Humans
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Leukemia-Lymphoma, Adult T-Cell / genetics*
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Leukemia-Lymphoma, Adult T-Cell / pathology
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Neoplasm Proteins / analysis
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Neoplasm Proteins / genetics
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Point Mutation*
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Translocation, Genetic*
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Tumor Cells, Cultured*
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Tumor Suppressor Protein p53 / analysis
Substances
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Codon
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Neoplasm Proteins
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Tumor Suppressor Protein p53